Localization of Glucose Oxidase and Catalase Activities in Aspergillus niger.
نویسندگان
چکیده
The subcellular localization of glucose oxidase (EC 1.1.3.4) in Aspergillus niger N400 (CBS 120.49) was investigated by (immuno)cytochemical methods. By these methods, the bulk of the enzyme was found to be localized in the cell wall. In addition, four different catalases (EC 1.11.1.6) were demonstrated by nondenaturing polyacrylamide gel electrophoresis of crude extracts of induced and noninduced cells. Comparison of both protoplast and mycelial extracts indicated that, of two constitutive catalases, one is located outside the cell membrane whereas the other is intracellular. Parallel with the induction of glucose oxidase, two other catalases are also induced, one located intracellularly and one located extracellularly. Furthermore, lactonase (EC 3.1.1.17) activity, catalyzing the hydrolysis of glucono-delta-lactone to gluconic acid, was found to be exclusively located outside the cell membrane, indicating that gluconate formation in A. niger occurs extracellularly.
منابع مشابه
University of Groningen Cytochemical Localization of Glucose Oxidase in Peroxisomes of Aspergillus niger
متن کامل
A discussion of anti-Aspergillus niger glucose oxidase monoclonal antibody reactivity to red blood cells of several species.
We observed that mouse spleen cells from rosettes with autologous red blood cells (RBCs) and that rosette-formation was suppressed by anti-Aspergillus niger glucose oxidase monoclonal antibody (mAb). In the present study, we investigated whether RBCs of species besides mice have the structure recognized by anti-A. niger glucose oxidase mAb by using rosette-formation and complement-mediated hemo...
متن کاملTHE EFFECT OF IONIC STRENGTH ON THE BINDING OF SODIUM N-DODECYL SULPHATE TO ASPERGILLUS NIGER CATALASE
The binding of sodium n-dodecyl sulphate (SDS) to Aspergillus niger catalase in various ionic strengths at pH 6.4 and a temperature of 27? and 37?C has been studied over a range of SDS concentrations using equilibrium dialysis and fluorescence spectroscopy techniques. The binding data were used and interpreted in terms of theoretical models (Scatchard Equation and Wyman Binding Potential). ...
متن کاملPurification and Properties of the Glucose Oxidase from Aspergillus Niger.
Glucose oxidase (/3-n-glucose : 02 oxidoreductase, EC 1.1.3.4) has been highly purified from the extracts of three fungi, Penicillium not&urn (1, 2), Penicillium amagasakiense (3, 4), and Aspergillus niger (5, 6). It has also been identified and sometimes partially purified from a number of other sources. Some of these sources are reviewed by Schepartz and Subers (7). Recently Pazur and Kleppe ...
متن کاملEffect of Gamma Irradiation on Aspergillus Niger for Enhanced Production of Glucose Oxidase
Developing countries have a high prevalence of diabetes and their populations are suffering from associated adverse factors. Such a frequency requires more effective diagnosis, mostly achieved by glucose diagnostic kits. Although high priced kits are available in market but local production of such kits can be highly cost effective and may confer the decline in incidence of the disease. Glucose...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 58 4 شماره
صفحات -
تاریخ انتشار 1992